yaaH [2019-08-15 09:45:20]

general stress protein, survival of ethanol stress, SafA-dependent protein of the inner spore coat, spore cortex lytic protein

Locus: BSU_00160

Isoelectric point: 5.62

Molecular weight: 48.47 kDa

Protein length: 427 aa

Gene length: 1284 bp

Function: survival of ethanol stress, protection of the spore

Product: N-acetylglucosaminidase

Essential: no

Synonyms: sleL

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Sporulation → Sporulation proteins → Spore coat proteins → Class I
• Lifestyles → Coping with stress → General stress proteins (controlled by SigB)

Gene

Coordinates: 23,868 25,151

The protein

Protein family

• Glycosyl hydrolase 18 family (according to UniProt)
• Chitinase class II subfamily (with YdhD, according to UniProt)

Paralogous protein(s)

• YdhD

Domains

• contains two N-acetylglucosamine-polymer-binding LysM domains at the N-terminus PubMed
• glycoside hydrolase family 18 domain (aa 125 to 410)

Structure

• 4S3K (SleL from B. megaterium, 55% identity) PubMed

Localization

• inner spore coat, localization depends on SafA PubMed

Additional information

• required for survival of ethanol stress PubMed

Expression and Regulation

Operons

• Genes: yaaH
  Description: PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon
  • SigB: sigma factor, PubMed, in SigB regulon

  Regulatory mechanism

  • SpoIIID: repression, PubMed, in SpoIIID regulon

  Regulation

  • expressed during sporulation in the mother cell (SigE, SpoIIID) PubMed

  Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed

• Genes: yaaI-yaaH
  Description: PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in SigB regulon

  Regulation

  • induced by stress (SigB) PubMed

Biological materials

Mutant

• MGNA-B889 (yaaH::erm), available at the NBRP B. subtilis, Japan
• BKE00160 (yaaH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACATAAATTTGAATGAAAA, downstream forward: _UP4_TAATTTGGAAACGTCTTTTT
• BKK00160 (yaaH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACATAAATTTGAATGAAAA, downstream forward: _UP4_TAATTTGGAAACGTCTTTTT

References

Reviews

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Original publications

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